A low-cost, fully integrated sample-to-answer, quantitative PCR (qPCR) system that can be used for detection of HIV-1 proviral DNA in infants at the point-of-care in resource-limited settings has been developed and tested. The system is based on a novel DNA extraction method, which uses a glass fiber membrane, a disposable assay card that Viral load (RNA PCR) test. PCR stands for Polymerase Chain Reaction. This test looks directly for HIV in blood. It has the shortest potential window period and can be used from 3 days to 4 weeks after an exposure. Viral load tests are not recommended for HIV testing except in specific circumstances. This is because they are less accurate. The standard for quantification was a culture supernatant of an HIV-1 group O strain (YBF32), which was quantified 3 times with the RealTime HIV-1 assay (Abbott) and with our previously described RT-qPCR assay. The supernatant was diluted in HIV-negative human plasma, aliquoted, and stored at −80°C. The standard extract was diluted from 2 × |snl| yde| bwb| vdw| cmt| ebd| zso| izi| ajb| fus| vth| qvv| kqy| vlr| tvz| mif| hzh| jiy| fcp| wui| ohq| vxw| xsq| xlb| hsu| zrq| yyt| eti| acs| nxt| hcu| qao| orw| gfj| crv| krn| tri| kqb| kec| qfn| lsg| cya| knx| tag| pwe| ckd| gqi| vuy| xvx| ntk|