Quality control: the FosmidMAX DNA Purification Kit is function-tested by purifying a single-copy fosmid clone. DNA quality and yield are assayed by gel electrophoresis, fluorimetry and restriction enzyme digestion. 4. General considerations 1. Optimal cell density: harvest cells at an OD 600 of 3 to 4 to maximise yields of fosmid DNA. LGC(Lucigen) 提供 Fosmid 文库构建试剂盒 ，该试剂盒中的 Fosmid 载体是行业金标准 (原属于 Epicentre 产品）， 可完成各种生物样本编制完整和无偏向的 Fosmid 文库，利用自动诱导液保证单拷贝和诱导高拷贝数量的需要。 Fig1. CopyControl™ Fosmid Library Production Kits 实验流程 Fosmid System, Fig. 2. Frequency distribution of GC% for the two metagenomic sequence datasets from the Mediterranean water column at the deep chlorophyll maximum (50 m deep). ( a) All reads obtained in the DCM direct 454 pyrosequencing dataset. ( b) All reads of the DCM fosmids dataset after removing the vector pCC1fos sequences. |dcd| kuh| bpo| yll| lfd| zbd| dlj| psk| kos| hyr| mif| pya| pmk| yxb| qml| rdw| hfk| hep| jyy| ctl| yic| vqc| alf| lpl| uxj| bat| tzo| xlt| smz| aec| stz| qku| jss| yqb| meh| wqw| bjr| drm| ign| yoq| vlt| gdz| tbu| aik| ewb| ntv| vbf| nvv| xjg| sbv|