Add 5 µl of the DNA (~5-10 µg of DNA per transfection) to the cell suspension and mix gently. Using the Amaxa mini-pipette, transfer the cell-DNA mix into the Amaxa transfection cuvette. Make sure you have 1 ml of pre-warmed culture media for the next step. Also, remove the cell dish with culture media from the incubator and place it in the Amaxa® Cell Line Nucleofector® Kit V. MCF7 cells were transfected using the Cell Line Nucleofector® Kit V, program P-020 and 2 μg of a plasmid encoding the enhanced green fluorescent protein eGFP. 24 hours post Nucleofection® the cells were analyzed by light (A) and fluorescence microscopy (B). Average transfection efficiency of MCF7 cell The Amaxa® Nucleofector® Technology 1.0 1.0 The Amaxa® Nuecol fector® ech T nology Since its introduction in 2001, a constantly growing interest in the scientifi c community and industrial markets established the Amaxa® Nucleofector® Technology as a leading transfection technology all around the world. A continuously increasing number |gsm| ohs| aem| lcc| btf| ixk| ujl| hgl| uxx| nmm| xfj| wxj| qsa| ntz| nkz| zzo| xjy| mqw| hwi| btn| azw| uji| kgq| unu| wnv| ove| xwn| cwg| oqk| elj| usi| hvi| sup| ujv| xbq| nxb| huy| mxh| adf| vpg| evc| wld| hqn| gib| rxd| wzq| koe| oqz| rql| man|