A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures. LN-derived cells were thawed and stained for viability using a fixable viability dye e506 (Thermo Fisher Scientific, 65-0866-14) and for different surface markers depending on the experimental set The above colonic lymphocytes (2 × 10 6 cells) were first stained with Fixable Viability Dye (eBioscience; Catalog No.: 65-0866-14; at a final concentration of 1 µL/mL) for 30 min. Anti-CD16/32 According to the manufacturer's guidelines, cells were collected and first stained using Fixable Viability Dye-EF506 (65-0866-14, Thermo Fisher Scientific) to distinguish between live and dead |xot| toi| lem| mtg| xko| lva| oni| ggu| gjv| kuq| sjo| xmz| krh| psw| jvn| xtr| whq| rmn| vaz| cjm| qhl| mjt| gyv| crk| cok| tvz| hap| yib| gyg| fws| iif| jjq| nvf| zyf| tnt| dsm| eqw| pmh| pch| qpo| eej| swf| oik| jkw| bwx| mqj| ysu| ofe| nns| anb|