Azoarcusのグループiのイントロンribozymeの医薬品
First, we show that RNA1 is elongated by multiple additions of small fragments in the presence of the ribozyme.RNA1 is composed of an 18 nt "stem" (S), a 3 nt tag, and a 5 nt "mobile unit" ().We observe a variety of products denoted S n, which length corresponds to the addition of n mobile units appended to the stem. Polyacrylamide gel images reveal four bands after 1 hour of reaction
Group II introns are mobile ribozymes that self-splice from precursor RNAs to yield excised intron lariat RNAs, which then invade new genomic DNA sites by reverse splicing. The introns encode a reverse transcriptase that stabilizes the catalytically active RNA structure for forward and reverse splicing, and afterwards converts the integrated
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